Recombinant Protein Expression & Production

Prokaryotic (E. coli) and eukaryotic (HEK293) expression routes, selected around your protein's folding and post-translational modification requirements — delivered with a Technical Report.

Two expression systems, chosen on the biology — not on convenience

Glycosylation, disulfide bonding and conformational complexity decide the host. We run both routes and recommend the one that gives you a functional antigen rather than merely a band of the right size.

3.1 Prokaryotic

E. coli expression

No glycosylation or other post-translational modification — suited to structurally simple proteins and antigen peptides.

3.2 Eukaryotic

HEK293 expression

Native glycosylation and folding machinery — suited to membrane-protein ectodomains and complex conformational antigens.

3.1

Prokaryotic protein expression

Recombinant protein expressed and prepared in an Escherichia coli (E. coli) expression system.

Item	Specification
Expression system	E. coli (prokaryotic)
Purity (reduced)	SDS-PAGE > 90%
Monomer purity	SEC-HPLC > 90%
Deliverables	Recombinant protein, Technical Report
Timeline	3–4 weeks from gene sequence to protein delivery

3.2

Eukaryotic protein expression

Recombinant protein expressed and prepared in a HEK293 cell expression system.

Item	Specification
Expression system	HEK293 (eukaryotic)
Purity (reduced)	SDS-PAGE > 90%
Monomer purity	SEC-HPLC > 90%
Deliverables	Recombinant protein, Technical Report
Timeline	3–4 weeks from gene sequence to protein delivery

Workflow — from gene sequence to delivered protein

A documented six-stage route, 3–4 weeks end to end.

Recombinant protein expression

3–4 weeks

Gene synthesis

Codon-optimised sequence

Vector construction

Expression vector assembly

Expression

E. coli or HEK293

Purification

Chromatographic capture

QC & release

Purity, SEC

Delivery

Protein & Technical Report

Quality standard

Purity and monomer content are tested against defined acceptance criteria and reported in the Technical Report.

Test item	Acceptance criterion	Method
Antibody purity (reduced)	> 90%	SDS-PAGE
Antibody monomer purity	> 90%	SEC-HPLC
Endotoxin	< 1 EU/mg	Limulus amoebocyte lysate (LAL)
Recombinant protein purity	> 90%	SDS-PAGE / SEC-HPLC
Supernatant antibody concentration	Quantitative report	Quantitative assay
Affinity	EC₅₀ determination	Concentration–binding curve fitting

Not sure which expression system fits your antigen?

Send us the sequence or the target biology. We will recommend the host, the tag strategy and a realistic timeline before you commit.

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